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اقسم بالله كان طلع بجديات تأجل معناها ربي يحبني وربي رقدت ثلاث مرات في القاعه 🤣🤣 واللي قدامي تنوض فيا مسكينه
TEM → inside
SEM → surface
LM → light + oil immersion ×100
والأرقام المهمة جدًا:
LM 0.1–0.2 µm — EM 0.1–0.2 nm — TEM 20–100 nm — LM objectives 3.5/10/40/100.
LM TEM SEM
Light Electrons Electrons
2D Interior Surface
Resolution أقل Resolution أعلى أقل من TEM
×100 oil immersion Sections 20–100 nm 3D
— Internal details Surface details
• 1. rER = proteins; sER = lipids/steroids/detox/Ca²■.
• 2. Golgi = modification + sorting + packaging.
• 3. Cis-Golgi receives from ER; trans-Golgi sorts/ships.
• 4. Lysosome = intracellular digestion.
• 5. Low pH activates lysosomal hydrolytic enzymes.
• 6. Phagocytosis = large particles/bacteria.
• 7. Pinocytosis = fluid/small molecules.
• 8. Receptor-mediated endocytosis = selective uptake.
• 9. Mitochondria = ATP; has circular mtDNA.
• 10. Inner mitochondrial membrane = cristae + ATP synthesis complexes.
• 11. Peroxisome = H■O■ + detox + β-oxidation.
• 12. Actin ≈7 nm; microtubules ≈25 nm; intermediate ≈10 nm.
• 13. Actin uses ATP; microtubules use GTP.
• 14. Kinesin → plus end; dynein → minus end.
• 15. Intermediate filaments are stable and provide strength.
• 16. Keratin → epithelial cells.
• 17. Desmin → muscle.
• 18. GFAP → astrocytes.
• 19. Neurofilaments → neurons.
• 20. Ribosomes translate mRNA.
• 21. Proteasomes degrade ubiquitin-tagged proteins.
• 22. Centriole = 9 microtubule triplets.
• 23. Nuclear envelope = two membranes.
• 24. Outer nuclear membrane continuous with rER.
• 25. Heterochromatin = condensed + inactive + dark; euchromatin = loose + active + pale.
1. Histology = study of tissues.
2. Cells → tissues → organs → systems
3. Tissue components = cells + ECM
4. Tissue culture = in vitro
5. Most common microtechnique = Paraffin
6. Most rapid = Freezing
7. Fixative = 10% formaldehyde + 90% normal saline
8. Fixation prevents autolysis + putrefaction
9. Dehydration = ascending alcohol concentrations
10. Clearing = Xylol
11. Impregnation = soft paraffin, 52–60°C
12. Embedding = hard paraffin
13. Sectioning = microtome
14. Hematoxylin = basic → basophilic → blue
15. Eosin = acid → acidophilic → red
16. Vital = in vivo
17. Supravital = in vitro
18. PAS = glycogen
19. Sudan = lipid
20. TEM = inside / SEM = surface 3D
