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Histopathology_And_Cytology_Final_Shot_By_Paramedical_Sarathi_.pdf
*Quality Control histopathology* 📚
91. *Most common artifact in histology → Tissue shrinkage*
92. *Formalin pigment → Acid formaldehyde hematin*
93. *Floater artifact → Cross-contamination tissue fragment*
94. *Knife lines → Nicks in microtome knife*
95. *Crush artifact → Due to forceps handling*
96. *Air bubble artifact → Poor mounting*
97. *Section folds → Improper stretching on water bath*
98. *Autolysis prevented by → Immediate fixation*
99. *Pigments like melanin can be bleached by → Hydrogen peroxide*
100. *Quality control in staining ensures → Diagnostic accuracy*
*Other Special Stains* 🩵
81. *Feulgen stain → DNA (magenta)*
82. *Methyl green-pyronin → DNA (green), RNA (red)*
83. *Toluidine blue → Mast cells (metachromasia)*
84. *Alizarin red S → Calcium deposits*
85. *Von Kossa stain → Calcium (black)*
86. *Schmorl’s stain → Reducing substances (blue-green)*
87. *Hall’s stain → Bile pigment*
88. *Fouchet’s stain → Bile pigment (green)*
89. *Fontana Masson stain → Melanin (black)*
90. *Perls’ stain also detects → Hemosiderin*
*Special Stains – Kidney & Muscle* 📕
75. *Nonspecific esterase → Monocytes*
76. *Jones silver stain → Basement membrane in kidney*
77. *Periodic acid silver methenamine → Glomerular basement membrane*
78. *Gomori’s trichrome → Muscle pathology*
79. *ATPase stain in muscle → Muscle fiber typing*
80. *NADH-TR stain → Mitochondria in muscle*
*Special Stains – Blood & Bone Marrow* 🔺
71. *Perl’s Prussian blue → Iron (blue)*
72. *Myeloperoxidase stain → AML diagnosis*
73. *Sudan Black B stain → Myeloid series cells*
74. *Naphthol-ASD-chloroacetate esterase → Granulocytes*
