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𝐁𝐢𝐨𝐭𝐞𝐜𝐡𝐧𝐨𝐥𝐨𝐠𝐲: IIT JAM, GATE & GATB

𝐁𝐢𝐨𝐭𝐞𝐜𝐡𝐧𝐨𝐥𝐨𝐠𝐲: IIT JAM, GATE & GATB

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🏛️ 𝐏𝐫𝐚𝐜𝐭𝐢𝐜𝐚𝐥/𝐋𝐞𝐜𝐭𝐮𝐫𝐞 𝐂𝐨𝐮𝐫𝐬𝐞, 𝐖𝐨𝐫𝐤𝐬𝐡𝐨𝐩, 𝐒𝐲𝐦𝐩𝐨𝐬𝐢𝐮𝐦, 𝐓𝐫𝐚𝐢𝐧𝐢𝐧𝐠, 𝐂𝐨𝐧𝐟𝐞𝐫𝐞𝐧𝐜𝐞𝐬, 𝐖𝐞𝐛𝐢𝐧𝐚𝐫, 𝐏𝐫𝐨𝐣𝐞𝐜𝐭𝐬, 𝐉𝐨𝐛𝐬 👨‍💻 𝐆𝐀𝐓𝐄, 𝐈𝐈𝐓𝐉𝐀𝐌, 𝐆𝐀𝐓𝐁 🎗️ 𝐏𝐫𝐨𝐦𝐨𝐭𝐢𝐨𝐧 @TheBookShelfBot

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A Plasmid has 4 restriction site 2 of EcoRI and and 2 of Xhol, in the distance of 2 kb, 2kb and 4kb and 5kb. Complete digestion with Xhol and EcoRI. How many bands you will get when observed in agarose gel?
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What is the isoelectric point (pI) of the amino acid arginine (Arg) if its pKa values are: carboxyl group (2.17), alpha-amino group (9.04), and side chain (12.48)?
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