علم الكيمياء ⌬ Chemistry Science

Peripheral Blood Film (PBF) Test 1. Objective The objective of this test was to examine the morphology of red blood cells, white blood cells, and platelets, in order to detect anemia, infections, leukemia, and other hematological disorders. 2. Principle The test was based on microscopic examination of a thin blood smear stained with a Romanowsky stain such as Leishman or Wright stain. Different blood cells took up the stain differently, allowing their size, shape, number, and internal structures to be clearly observed under a microscope. 3. Materials Fresh capillary or venous blood sample Clean glass slides Spreader slide Leishman or Wright stain Buffer solution or distilled water Dropper Microscope Immersion oil Gloves and laboratory safety equipment 4. Procedure A small drop of fresh blood was placed near one end of a clean glass slide. Another slide was used as a spreader and held at an angle of about 30–45 degrees. The blood was spread smoothly to make a thin smear. The smear was air-dried completely. The dried smear was stained with Leishman or Wright stain for the required time. Buffer or water was added to dilute the stain and the slide was washed gently. The slide was air-dried and examined under the microscope using low power and oil immersion objectives. 5. Result Red blood cells showed normal or abnormal size, shape, and color as observed. White blood cells were identified and differentiated into neutrophils, lymphocytes, monocytes, eosinophils, and basophils. Platelets were seen as small granular bodies. (Example: Normocytic normochromic RBCs; normal WBC differential; adequate platelets.) 6. Uses It was used to diagnose different types of anemia. It helped in detecting infections and inflammatory conditions. It assisted in identifying leukemia and other blood disorders. It was useful for evaluating platelet abnormalities. 7. Conclusion The peripheral blood film test was successfully performed. Microscopic examination of the stained blood smear provided valuable information about blood cell morphology and helped in the diagnosis and monitoring of hematological disorders. #اختبار #تحليل #test