Group( D )

.......Presence of G or C bases within the last five bases from the 3' end of primers.

It is generally accepted that the optimal length of primers is.....

...... (ex: ATATATAT)>misprime.

Must be complementary to flanking sequences of target region.

A primer with a mismatch at the 3' end can still amplify DNA efficiently in SNPPCR.

The annealing step in PCR occurs at the highest temperature.

SNP-PCR can detect single nucleotide variations in DNA. مهمه

What is the role of primers in PCR?مهمه

Which reagent is essential for the extension step of PCR?مهمه

. What is the minimum population frequency required for a nucleotide variation to be classified as an SNP? مهمه

What is the primary purpose of PCR? مهمه

• In only 20 cycles, PCR can product about a million (2²0) copies of the target.

The reaction is heated to a temperature typical ......for efficient DNA synthesis ?

....... The reaction is heated to a temperature depends on the DNA polymerase used.

The reaction is rapidly cooled to the primer annealing temperature.....مهمه

The double-stranded template DNA is denatured by heating, typically to....

there is only one primer, only one strand of the double stranded DNA will amplified in pcr

........., which have the same sequence of reverse DNA strand and bind to thecomplementary forward strand.

......which have the samesequence of forward DNA strand and bind to the complementary reverse strand.

PCR does not copy all of the DNA in the sample. It copies only a very specific sequence.