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11:11 اللهم انت ولي نعمتي والقادر على طلبتي

اليوم 11/11 انتظرو من تصير ب11:11 م احس هذا اليوم بؤرة الطاقة الايجابية 😍😍 ترفع فيها رسائل الى السماء 💛 حضرو امنياتكم 💛

#بكتريا 👆👆👆 #مايكرو

Bacillus : Anthrax -G(+) -spore (oval, central, nonbulging ) -capsulated -catalase (+) -non motile -Blood agar (non haemolysis , Medusa head appearance) -spore stained by (malachite green and safranin)

LISTERIA : -G(+) -motile at 25 C° . nonmotile at 37C° -Mc agar : no growth -Blood agar : •L.monocytogens : narrow zone B-haemolysis •ivanovii : wider zone B-haemolysis CAMP : •L.monocytogenes (+) with S. aureus (SA) •L. ivanovii (+) with R. equi (RE). Anton test - Pathogenicity test in rabbit to confirm virulence - Virulent strains induce keratoconjuctivitis serology : not usefull (positive titer in normal animals and cross reaction with another G+ bacteria. Like S.aureus… )

Klebsiella : -G(-) -nonmotile -colony morphology : (On blood agar and Mc agar is mucoid colony (vascosity test) . -IMViC (- - + + ) ☻ عكس ايكولاي -citrate (+) - Urease : (+) -TSI :A/A proteus : -G(-) -motile -indole • P.mirabilis (-) • P.vugaris (+) -swarming on agar. -urease (+) - TSI : k/k + H2S production

Clostridim C.Tetani : -G(+) -motile -spore : drum stick shape 🥁 (oval,termnal, bulging) -Blood agar : beta-haemolysis -cooked meat agar swarming -strict anaerob C. botulinum : -fastidiius -strict anaerob -spore : oval subtermnal bulging -Blood agar : beta-haemolysis -motile C.perfringens : -non motile -capsulated -Spore (oval, subtermnal , non bulging ) - Blood agar ( double zone haemolysis ) - litmus milk (stormy reaction) {Ferment lactose (acid production), color changed from blue to pink.Coagulation of milk casein by acid, and clotted milk is disrupted by gas production} - Nagler’s reaction (growth of C.perfringens in serum or egg yolk agar producing turbidity around the colonies due to production of a-toxin (phospholipase C) )

Salmonella and Shigella Gram stain : -G(-) -Short rod -non capsule -not motile -no spores • Mc agar : NLF •IMViC (+ + - -) Selective media : 1•SS agar : --salmonella ⇨pale with black center . -- shigella ⇨ pink . 2•XLD :☻ مبينه 3•Chrome agar : salmonella (purple) TSI : - salmonella : K/A + H2S Production -shigella : K/A

E.coli : Mc agar : Selective to G(-) entirobecteriacea Differential to lactose fermenter (pink) and non lactose fermenter (colorless) E.coli colony is ( dry, pink 'lactose fermenter' ) on Mc agar . On EMB : green metallic sheen . Biochemical tests : - ferment (glucose , lactose , fructose) - IMViC (+ + - -) - indole (+) - methyle red test (+) - simmons citrate (-) - vogas-proskauer test (-) - motile TSI : A/A + Gas production

MYCOBACTERIUM Grow on Löwenstein–Jensen medium grow : - Slow-growing (2–6 weeks ) at 37°C. - colony morphology ‘ rough, tough and buff ’ colonies (__rough__ due to dry, tough due to difficulty in lifting the colony from the surface ; and buff due to the pale yellow color) ACID FAST STAINS • Ziehl-Neelsen – uses heat to drive the color into the lipids of the cell wall; decolorized with acid-alcohol • Kinyoun – cold stain . ZIEHL-NEELSEN ACID-FAST STAINING PROCEDURE : 1.fixation by heat . 2.add carbol fuchsin stain. 3.Heat the slide until it steams (5 min). 4.Pour off the carbol fuchsin. 5.Wash slide with water. 6.Decolorize with acid-alcohol (5 min). 7.Wash slide with water. 8.add methylene blue counterstain for 1 min. 9.Wash with water. 10. dry with bunsen flame. Positive Result is: The acid-fast bacilli are red color rods.

Corynebacterium • growth on Loeffler's medium . Stained by Albert’s stain : appear as metachromatic appearance ( Cytoplasm light green, granules blue-black) Mechanism of Tellurite Blood Agar • Reduction By Bacteria • Tellurium Precipitation • Black Colonies

Streptococcus Mc agar : no growth catalase : (-) Blood agar : alpha : -viridins -Pneumonia (optochin sensitive ) Beta : - pyogens (Bictracin sensitivite) - agalactia (CAMP test (+) ) Gamma : - entirococci -nonentirococci ☻ سنستڤ يعني اكو inhibition zone

Staphylococcus : Catalase (+) Mc agar : not growth MSA : selective for Ⓖ+ Differential for staphylococcus spp. S.aureus : mannitol fermenter (yellow) , caugulase (+) , Beta haemolysis on blood agar. S.epidermes : mannitol non fermenter (red) , caugulase (-) , no haemolysis on blood agar .

E.coli : Mc agar : Selective to G(-) entirobecteriacea Differential to lactose fermenter (pink) and non lactose fermenter (colorless) E.coli colony is ( dry, pink 'lactose fermenter' ) on Mc agar . On EMB : green metallic sheen . Biochemical tests : - ferment (glucose , lactose , fructose) - IMViC (+ + - -) - indole (+) - methyle red test (+) - simmons citrate (-) - vogas-proskauer test (-) - motile TSI : A/A + Gas production