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### Multiple Choice Questions 1. In traditional histology technique, what is used to harden tissues before cutting? a) Water b) Freezing c) Paraffin d) Epoxy resin 2. What is the typical thickness range for sections cut in traditional histology? a) 1-5 µm b) 2-50 µm c) 60-100 nm d) 0.5-1 µm 3. Which technique is known for its speed, taking only 15 minutes? a) Traditional histology b) Cryosectioning c) Electron microscopy d) Botanical microtomy 4. Cryosectioning is advantageous because it: a) Uses paraffin to harden tissues b) Requires 16 hours to process c) Prevents tissue degradation faster than fixatives d) Masks the chemical composition of tissues 5. In electron microscopy, tissues are embedded in: a) Paraffin b) Epoxy resin c) Frozen water d) Heavy metal salts 6. The thickness of sections cut for transmission electron microscopy is typically: a) 2-50 µm b) 0.5-1 µm c) 60-100 nm d) 100-200 nm 7. What kind of knife is used in an ultramicrotome for thin sectioning? a) Steel b) Glass c) Gem grade diamond d) Ceramic 8. Survey sections for TEM are typically of what thickness? a) 60-100 nm b) 2-50 µm c) 0.5-1 µm d) 15-20 µm 9. Botanical microtomy is particularly used for: a) Water-rich tissues b) Hard materials like bone and leather c) Soft tissues d) Chemical analysis 10. The main difference between cryosectioning and traditional histology is: a) The use of paraffin b) The use of freezing c) The use of heavy metal salts d) The use of epoxy resin 11. Which technique is typically used to achieve quick diagnosis during medical procedures? a) Traditional histology b) Cryosectioning c) Electron microscopy d) Botanical microtomy 12. What staining method is commonly used in electron microscopy? a) Aqueous dye b) Heavy metal salt c) Frozen dye d) Paraffin dye 13. The instrument used in electron microscopy for thin sectioning is called: a) Microtome b) Freezing microtome c) Sledge microtome d) Ultramicrotome 14. In traditional histology, paraffin is removed before staining because: a) It alters the tissue's chemical composition b) It makes the tissue too hard c) It prevents the stain from adhering d) It degrades the tissue 15. Which technique is most likely to be used for immunohistochemistry? a) Traditional histology b) Cryosectioning c) Electron microscopy d) Botanical microtomy ### True/False Questions 1. Traditional histology involves hardening tissues with freezing techniques. 2. Cryosectioning is faster than traditional histology. 3. Electron microscopy uses epoxy resin for embedding tissues. 4. Survey sections in electron microscopy are typically 60-100 nanometers thick. 5. Botanical microtomy is used for cutting thin sections of water-rich tissues. 6. The ultramicrotome is used in both electron microscopy and traditional histology. 7. Cryosectioning prevents tissue degradation better than traditional fixatives. 8. Traditional histology sections are stained without removing paraffin. 9. Heavy metal salts are used for staining in electron microscopy. 10. A sledge microtome is typically used for hard materials like bone and leather. These questions cover various aspects of the different histology techniques described in the text.

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