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Active site can bind to Substrate, Cofactors, competitive inhibitors.

Write the main mechanism of Enzyme Catalysis Proximity and Orientation, Bond strain , Covalent Catalysis , Acid-base Catalysis.

Write Types of Reversible enzymes inhibitors Competitive, Non Competitive, Uncompetitive.

Dental example of irreversible inhibitors Fluoride (high Concentration)

Low Km needs less substrate

Reaction rate is directly proportional to enzyme concentration.